Neutral amino acid transport systems in Chinese hamster ovary cells.
نویسندگان
چکیده
منابع مشابه
Neutral amino acid transport systems in Chinese hamster ovary cells.
The neutral amino acid transport systems A, ASC, and L have been characterized in the Chinese hamster ovary cell. System A, defined by its sodium ion dependency and inhibition by 2-methylaminoisobutyric acid, was found to be extremely sensitive to the pH of the external medium and to increase in response to starvation for amino acids. System ASC, identified by its sodium ion dependency and into...
متن کاملChemical modification of the neutral amino acid transport system L of Chinese hamster ovary cells with p-chloromercuribenzene sulfonate.
Branched-chain and aromatic neutral amino acids enter mammalian cells predominantly through a Na(+)-independent transport agency called System L. The sulfhydryl specific reagent p-chloromercuribenzene sulfonate (pCMBS) has been shown to be a potent inactivator of System L transport activity in Chinese hamster ovary cells, however, inactivation by pCMBS can be prevented by the presence of System...
متن کاملEvidence for a regulatory element controlling amino acid transport system L in Chinese hamster ovary cells.
We have used the technique of somatic cell hybridization to study the regulation of the neutral amino acid transport system L in Chinese hamster ovary (CHO) cells. The cell line CHO-ts025C1 has a temperature-sensitive mutation in leucyl-tRNA synthetase. At the nonpermissive temperature of 39 degrees C, CHO-ts025C1 cells are unable to charge leucyl-tRNA and behave as though starved for leucine b...
متن کاملRegulation of amino acid transport system L in Chinese hamster ovary cells.
The regulation of the activity of transport System L for neutral amino acids has been investigated in Chinese hamster ovary cells. Incubation of the temperature-sensitive leucyl-tRNA synthetase mutant CHO-tsH1 at marginally permissive temperatures results in leucine-limited growth and increased transport of branched chain and aromatic amino acids. This temperature-dependent transport enhancemen...
متن کاملDynamic profiling of amino acid transport and metabolism in Chinese hamster ovary cell culture
Introduction Chinese Hamster Ovary (CHO) cells are the most widely used industrial hosts for the production of recombinant DNA technology drugs [1]. In such processes amino acids (a.a.) are vital nutrients for growth, but also building blocks of the recombinant protein (rprotein). Our research aims to establish a better understanding of a.a. transport in and out of cells, since this could have ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1981
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)69218-5